抄録
This is a kind of review paper to introduce how our developing methodology for loss of heterozygosity (LOH) analysis is predictable to detect the biological effects of space radiation. Our biological experiments using human cultured cells in “Kibou” of International Space Station are planned to detect the following effects: i) mutagenic effects, ii) combined effects of radiation with micro gravity, and iii) radioadaptive response. After the cell recovery to the earth, we will analyze the mutagenic effects (i) in the frozen cells exposed to low-dose space radiation during the 3 months preservation in “Kibou”, determine the combined effects (II) from the mutation inductions in the cells incubated for 1 week under 1G or G in “Kibou”, and estimate the radioadaptive response (iii) from measuring the repair efficiencies of DNA double-strand breaks introduced after the recovery.
