HPLC/Chemiluminescence Detection of Methamphetamine and Amphetamine in Hair

抄録

  We have developed a highly sensitive HPLC method using chemiluminescence detection (CL-HPLC) for determining methamphetamine (MA) and its metabolite, amphetamine (AP), in a single hair. MA and AP were extracted from a single hair with a solution of methanol and hydrochloric acid, evaporated to dryness, dansylated, and subjected to CL-HPLC. Bis(2,4,6-trichlorophenyl) oxalate and hydrogen peroxide were used as post column reagents. This method was used for the determination of MA and AP in hair samples obtained from 44 MA users. Of these, 31 samples showed both MA and AP, 8 samples showed only MA and 5 samples showed neither MA nor AP. Seven MA users whose hair samples contained both MA and AP, neither compound was detected in the urine. Because the present method can be performed on a single hair, it is easier to procure samples and to obtain the subjects' willingness to provide samples.