抄録
When macrophages were incubated with VLDL-tri[1-14C] oleate (VLDL-TG-[14C]) and lipoprotein lipase (LpL), incorporation of lipid into macrophages was increased compared with in the absence of LpL. These results suggest that the metabolites of VLDL, especially FFA, might be easily incorporated into macrophages. But the calculated incorporation of metabolites except for FFA was also increased. These results indicate that the increase in incorporation into macrophages was due to the increase in incorporation of IDL or other altered lipoproteins. In the metabolism of FFA in the cells, cholesterol ester synthesis was increased by addition of LpL. The medium, which was incubated with VLDL-TG-(14C) in the presence or absence of LpL for 24 hours, was incubated with smooth muscle cells (SMC) for 24 hours. The medium which was derived from the condition with VLDL and LpL increased the incroporated radioactivity into SMC. In intracellular metabolism of lipid incorporated into cells, increase in phospholipid synthesis and decrease in cholesterol ester syntehsis were observed in the presence of LpL. These results suggest that the conditioned medium with LpL and macrophage regulated the incorporation of lipid into SMC and might regulate the metabolism in SMC.
