虚血性心臟病におけるコレステロール負荷時の血清リポ蛋白変動(第2報)

―特にアポ蛋白Eの変化について―

抄録

Seven hundred fifty mg of cholesterol were fed daily to 32 patients of the ischemic heart disease (IHD) for 2 weeks. Cholesterol amounts in VLDL, LDL, HDL, HDL₂ and HDL₃ were estimated on the 0th, 7th and 14th days of cholesterol load. Plasma apoprotein A-I, A-II, B, C-II, E levels were also measured in 16 from 32 IHD subjects. Apoprotein levels were estimated by a method of single radial immunodiffusion (SRID). Before cholesterol administration, cholesterol amounts in VLDL, LDL, HDL, HDL₂ and HDL₃ were 15.9±7.5mg/dl (mean±SD), 150.9±58.1mg/dl 43.8±9.6mg/dl, 16.4±5.8mg/dl and 24.9±6.3mg/dl respectively. Plasma apoprotein A-I, A-II, B, C-II and E levels were 81.7±22.0 mg/dl, 15.1±5.4mg/dl, 93.7±36.0mg/dl, 3.83±1.00mg/dl and 4.29±1.36mg/dl respectively. After 2 weeks' cholesterol feeding, all plasma lipoprotein cholesterol and apoprotein levels did not change significantly. Correlation coefficients between plasma apoprotein and lipoprotein cholesterol levels were calculated. Apoprotein A-I and A-II correlated with HDL-C (r=0.348), HDL₂-C (r=0.612) and HDL₃ (r=0.569). Apoprotein B correlated with total cholesterol (TC) (r=0.610), VLDL-C (r=0.341), LDL-C (r=0.726), HDL-C (r=0.432) and HDL₂-C (r=0.465). Apoprotein C-II correlated with TC (r=0.765), TG (r=0.679), VLDL-C (r=0.651), LDL-C (r=0.461) and HDL₂-C (r=0.326). Apoprotein E correlated with TC (r=0.580), TG (r=0.575) and VLDL-C (r=0.666). Interapoprotein's correlations were also calculated. Apoprotein A-I correlated with apoprotein A-II (r=0.468). Apoprotein B correlated with apoprotein C-II (r=0.393). Apoprotein C-II correlated with apoprotein B (r=0.393) and E (r=0.549). Apoprotein E only correlated with apoprotein C-II (r=0.549).