ヒト単球由来マクロファージにおける生体由来過酸化LDLの代謝

抄録

It is well known that low density lipoproteins (LDL) modified in vitro is mainly metabolised in Scavenger cells such as macrophage (Mφ) which could be developed to foam cell. However, there have been no reports concerning the metabolism of LDL modified in vivo in Mφ.
Thus, the present study was undertaken to clarify whether the modified LDL is catabolized in human monocyte-delived Mφ in vitro.
The LDL was obtained from hemodialysis (HD) patients who have usually high concentration of serum malondialdehyde (MDA) compared with those of normal controls. In addition, the reduction of the patients MDA was due to the administration of a-Tocopherol (TOC) at a dose of 600mg per day.
The Binding, incorporation and degradation of LDL were carried out according to the methods of Goldstein and Brown.
MDA levels in LDL were significant higher in HD-patients than controls. TOC-treatment slightly reduced LDL-MDA levels in HD-patients.
In binding and uptake, there were no significant differences between patients and controls. On the other hand, there was the significant increase of degradation in patients and the change was slightly recovered toward the degradation of controls.
In conclusion, it was suggested that MDA-rich LDL obtained from in vivo as well as artificial MDA-rich LDL (in vitro) may receive the accelation of degradation in M φ.