The changes in the chemical components of subfractionated lipoproteins were estimated after plasmapheresis up to 28 days. The effects of probucol, cholestyramine, CS-514 on the chemical compositions of lipoploteins after plasmapheresis were also investigated.
Six FH patients and one non-familial type IIb hyperlipidemic subject were treated. Two to three litters of plasmas were treated at each plasmapheresis by double filtration or dextran-sulfate cellurose column method. Probucol (1g/day), cholestyramine (12g/day) or CS-514 (10-40mg/day) was administered after plasmapheresis.
TG increased in every lipoprotein fractions immediately after plasmapheresis. VLDL and IDL became cholesterol-rich and tryglyceride-poor molecules gradually without medication. The chemical compositions of LDL1 and LDL2 were relativery constant throughout 28 days after plasmapheresis. HDL2 became triglyceride-rich and apo A-I-rich, and HDL3 became apo A-I-poor immediately after plasmapheresis and return to the same chemical compositions as those of preplasmapheresis. Probucol prevented cholesterol increase in VLDL and IDL. Apo B in LDL1 and LDL2 increased under probucol treatment. HDL2 became cholesterol-rich and apo A-I poor molecules under probucol treatment. Probucol increased apo A-I in HDL3. Cholestyramine had almost no effects on the changes in chemical compositions of plasma lipoproteins after plasmapheresis. CS-514 prevented VLDL-cholesterol increase but had almost no effects on the changes in chemical compositions of other lipoprotein fractions after plasmapheresis.
