抄録
Recently we have reported that an impairment of very low density lipoprotein (VLDL)-triglyceride (TG) catabolism is responsible for elevated plasma TG concentration in puromycin-induced nephrotic rats and that the half life of VLDL-TG from nephrotic rats in plasma was significantly longer than that from normal rats reinjected in normal recipient rats. We therefore analyzed the composition of VLDL in nephrotic rats to determine what's compositional change of VLDL particles may be associated with the defect in VLDL-TG catabolism. Polyacrylamide gel electrophoresis revealed that the relative proportion of apoprotein (apo) B 100+95/48 was significantly increased and the relative ratio of apo E to apo C was significantly decreased on nephrotic VLDL particles. No significant changes of apo C subspecies between nephrotic and normal VLDL was observed by the analysis of isoelectric focusing gel. The ratio of TG or cholesterol to apoprotein in VLDL was significantly increased in nephrosis, suggesting a larger particle size than the normal. These results suggest that compositional abnormalities of apoproteins and lipids is partially associated with the delayed clearance of nephrotic VLDL in plasma circulation.
