モルモット気道上皮の杯細胞のペルオキシダーゼ活性に関する電顕組織化学的研究

抄録

The distribution and localization of endogenous peroxidase activity in the tracheobronchial epithelium of guinea pigs were investigated with both light and electron microscopes using a cytochemical procedure of the diaminobenzidine-H₂O₂ method after Graham and Karnovsky. Male guinea pigs were used at the following time-intervals after birth; 2 days, 4 days, 2 weeks, 1 month, 2 months and 6 months to 2 years
The following results are obtained;
1) Goblet cells in the tracheobronchial epithelium were cytochemically subdivided into two types of cells, that is, the peroxidase-positive cells and the peroxidase-negative cells. Both types of cells were provided with mucous granules. They independently possess the complete secretory function.
2) The peroxidase-positive goblet cells were characterized by the presence of reaction product in the r-ER, perinuclear space and some of the Golgi complexes. However, no reaction product was discernible in the entire cytoplasm of the peroxidase-negative cells.
3) The peroxidase-positive goblet cells increased with an inverse relation to the peroxidase-negative goblet cells as the animals grew older. Most of the goblet cells in the main bronchi of 2 to 4-day-old guinea pigs were peroxidase-negative, whereas nearly all of the goblet cells of 6 month to 2-year-old guinea pigs were positive.
4) The peroxidase-positive goblet cells appeared to be more dominant in the upper trachea than in the main bronchi.
5) Reaction product localized in the r-ER, s-ER, perinuclear space and the Golgi complex was also noted in the ciliated cells. Ciliated cells with cytochemical reaction product were consistently distributed throughout the epithelium of the lower respiratory tract.
6) The peroxidase activity in the goblet cells and ciliated cells was clearly inhibited with 0.02M 3-amino-1, 2, 4-triazole or 0.02M potassium cyanide.