The authors prepared a dual-phase asthmatic response model (DAR), an immediate asthmatic response model (IAR) and a continual asthmatic response model by sensitizing Hartley-strain male guinea pigs. The purpose was to study serial changes to T-cells in the bronchial tissue of each model with fluorescence stains using either anti-CD4 antibodies or anti-CD8 antibodies. Histologically, in the DAR group marked CD4+ cells were observed at 4 h after contact with aerosolised OA, which decreased after 24 h. CD8+ cells incresed at 24 h after contact in the bronchial walls of the DAR group. In IAR group, a few CD4+cells were seen 4 h after contact. In the continual asthmatic response group, marked CD4+cells were observed 4 h after contact, and infiltrates of CD4+ cells were not seen to decrease at 24 h after contact- Further, the DAR group received either an anti-CD4+ antibody or an anti-CD8+antibody intraperitoneally 30 min before contact. The depletion of CD8+cells in the model indicates the presence of DAR. In the asthma model, CD4+ T cells inhabited the LAR after antigen inhalation.
