A 57-year-old woman visited our hospital complaining of a dyspnea feeling. A tumor was revealed in the posterior wall of the trachea by laryngoscopy. The tumor was shown to have a developed from the trachea by CT and MRI findings. Since a biopsy from the tumor by tracheobronchoscopy for pathological diagnosis poses certain risks, such as bleeding and dyspnea, fine needle aspiration biopsy under ultrasonography was first performed, and the pathological diagnosis was adenoid cystic carcinoma. After intubating the trachea with a thin intubation tube for general anesthesia, the tracheostomy was performed, and then the procedure was switched to intratracheal intubation a with normal-sized tube. A tracheo-laryngectomy was performed with a safety margin. This suggests that fine needle aspiration biopsy can be a safe and useful method for tracheal tumors.