Journal of Biological Macromolecules
Online ISSN : 2187-3240
Print ISSN : 1347-2194
ISSN-L : 1347-2194
Specific gene expression analysis in Aspergillus oryzae grown in wheat bran without starch.
Akihiro Kaneko Motoaki SanoShinichi OhashiKenji Ozeki
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ジャーナル オープンアクセス

2014 年 14 巻 1 号 p. 15-26

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抄録
A starch removal method from a wheat bran medium was established using citric acid. Secreted enzymes from cultivated A. oryzae RIB40 were then investigated. The specific activity of α-amylase decreased while that of xylanase and xylosidase increased 2-fold and 7-fold, respectively. Proteomics analysis identified three new α-glucosidases and two new α-glucanases. DNA microarray identified high expression of three new endo glucanases and one α-xylosidase. The expression of β-1,4 xylanase was increased 27-fold.
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© 2014 Japan Science Society of Biological Macromolecules
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