Preparation of gelatin scaffold and fibroblast cell culture

Yoshinori Takagi; Susumu Tanaka; Syo Tomita; Shuri Akiyama; Yasuyuki Maki; Takao Yamamoto; Masumi Uehara; Toshiaki Dobashi

doi:10.17106/jbr.31.2

BRIEF COMMUNICATION

Preparation of gelatin scaffold and fibroblast cell culture

Yoshinori Takagi, Susumu Tanaka, Syo Tomita, Shuri Akiyama, Yasuyuki Maki, Takao Yamamoto, Masumi Uehara, Toshiaki Dobashi

著者情報

キーワード: fibroblast, cell culture, gelatin gel scaffold, UV-irradiation

ジャーナルフリー

2017 年 31 巻 1 号 p. 2-5

DOI https://doi.org/10.17106/jbr.31.2

詳細

抄録

A gelatin gel sheet was UV-irradiated to raise the gel melting temperature T_m. Three second of T_m increased proportionally to irradiation time t_UV. The sheet of which T_m was larger than the culture temperature 37°C was used as a cell culture scaffold. Human fibroblast cells WI-38 adhered to the sheet and dug deeply into it regardless of t_UV, while cancer cells WI-38-VA-13 formed spheroids and did not adhere to the sheet individually at high UV dose. The cell selectivity of the scaffold could be used for biological and medical studies.

責任著者(Corresponding author)

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