Preparation of gelatin scaffold and fibroblast cell culture

Yoshinori Takagi; Susumu Tanaka; Syo Tomita; Shuri Akiyama; Yasuyuki Maki; Takao Yamamoto; Masumi Uehara; Toshiaki Dobashi

doi:10.17106/jbr.31.2

BRIEF COMMUNICATION

Preparation of gelatin scaffold and fibroblast cell culture

Yoshinori Takagi, Susumu Tanaka, Syo Tomita, Shuri Akiyama, Yasuyuki Maki, Takao Yamamoto, Masumi Uehara, Toshiaki Dobashi

著者情報

Yoshinori Takagi
Division of Molecular Science, Graduate School of Science and Technology, Gunma University
Susumu Tanaka
Department of Health and Nutrition, Faculty of Health and Welfare, Takasaki University of Health and Welfare
Syo Tomita
Division of Molecular Science, Graduate School of Science and Technology, Gunma University
Shuri Akiyama
Department of Health and Nutrition, Faculty of Health and Welfare, Takasaki University of Health and Welfare
Yasuyuki Maki
Division of Molecular Science, Graduate School of Science and Technology, Gunma University
Takao Yamamoto
Division of Pure and Applied Science, Graduate School of Science and Technology, Gunma University
Masumi Uehara
Graduate School of Radiological Technology, Gunma Prefectural College of Health Sciences
Toshiaki Dobashi
Division of Molecular Science, Graduate School of Science and Technology, Gunma University

キーワード: fibroblast, cell culture, gelatin gel scaffold, UV-irradiation

ジャーナルフリー

2017 年 31 巻 1 号 p. 2-5

DOI https://doi.org/10.17106/jbr.31.2

詳細

抄録

A gelatin gel sheet was UV-irradiated to raise the gel melting temperature T_m. Three second of T_m increased proportionally to irradiation time t_UV. The sheet of which T_m was larger than the culture temperature 37°C was used as a cell culture scaffold. Human fibroblast cells WI-38 adhered to the sheet and dug deeply into it regardless of t_UV, while cancer cells WI-38-VA-13 formed spheroids and did not adhere to the sheet individually at high UV dose. The cell selectivity of the scaffold could be used for biological and medical studies.

責任著者(Corresponding author)

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