Growth Factors of A Yeast-Lysing Microorganism in Activated Sludge

Studies on Yeast-Lysing Microorganisms (Part II)

Abstract

Growth factors of a yeast-lysing microorganism (YLM-1) in activated sludge extrct were investigated.
Magnesium and nitrogenous compounds such as ammonia and amino acids except sulfer containing amino acids were necessary for growth of YLM-1.
Sulfur containing amino acids, fatty acids (C₃-C₄) and Ca showed inhibition for the growth of YLM-1.
From these results, media for YLM-1 were proposed as follows:
K₂HPO₄; 7.74g, MgSO₄.7 H₂0; 20.5mg, NH₄H₂PO₄; 115mg (or Glycine; 75 mg), yeast cell
suspension; 2×10¹¹ cells, distilled water; 1 litre, pH; 8.0