Growth factors of a yeast-lysing microorganism (YLM-1) in activated sludge extrct were investigated.
Magnesium and nitrogenous compounds such as ammonia and amino acids except sulfer containing amino acids were necessary for growth of YLM-1.
Sulfur containing amino acids, fatty acids (C
3-C
4) and Ca showed inhibition for the growth of YLM-1.
From these results, media for YLM-1 were proposed as follows:
K
2HPO
4; 7.74g, MgSO
4.7 H
20; 20.5mg, NH
4H
2PO
4; 115mg (or Glycine; 75 mg), yeast cell
suspension; 2×10
11 cells, distilled water; 1 litre, pH; 8.0
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