Analysis of the Methodology of Rabbit's Brain Tissue Transplantation Experiment

抄録

Objective: Severe cerebral contusion will cause a functional defect in partial central nerve and will lower patients' living quality. The above problem is still a difficult problem unsolved in clinical treatment. This study is going to clarify the methodology of rabbit's brain tissue transplantation, including the way of operation and the law of microcirculatory formation. Method: Twenty healthy male, Japanese white rabbits with long ears (about three months after birth) of clean degree, weighting between 1.2kg and 1.3kg, were anaesthetized by 3% pentobarbital sodium in vein before receiving an intracerebral transplantation operation. A window was opened on their parietal bone symmetrically, and the cortical brain tissues on the symmetrical areas (on the left and right side) of the rabbits' parietal cortex areas were exchanged and transplanted. Gentamycin sulfate was injected each day to resist infection. Ten and twenty days later, an observation was made as to the survival of the transplanted area and host brain tissue. A microcirculation color camera system was used to analyze the pictures of angiogenesis. With regard to the survival of transplanted brain tissues, their changes in micromorphology were observed. Besides, pathological sections were also prepared to determine their surviving conditions on cell level. Results: (1) Surgical operation has contributed to a satisfactory morphological anastomosis between transplanted brain tissues and host brain tissues. (2) Micro-blood vessel loops were observed to have formed on the section (host side) of rabbits' parietal cortex areas, which had been excised partially when transplantation was not filled. Marker was implanted into the transplanted brain tissues to confirm the possibility of the regeneration of a microcirculation among brain tissues, which had been exsomatized completely. (3) Analysis of the pathological sections of the transplanted brain tissues showed traces of surviving nervous cells. Conclusions: Under given conditions, nervous cells' survival can be maintained by transplanted brain tissues and can be nourished by angiogenesis and characteristic microcirculation connections with host brain tissues.