Explore the method of observing dynamic changes in microcirculation of rabbit's duodenal mucosal villi with a red color coordinate following brainstem hemorrhage

抄録

Objective Those who suffer from brainstem hemorrhage complicated by stress ulcer hemorrhage have a high mortality. This research inquire the ideal surgical method to observe microcirculation of duodenal mucosal villi and the dynamic changes in the microcirculation of rabbit's duodenal mucosal villi under brainstem hemorrhage conditions with a red color coordinate, aiming at establishing a mechanism for testing the microcirculation of the digestive tract and providing a reference index for the prevention and treatment of brainstem hemorrhage complicated by gastrointestinal bleeding. Method In this experiment, thirty male Japanese white rabbits with long ears (1.5kg to 1.8kg) were anaesthetized by 25% Urethane in vein, and simple disinfected in the condition of animal laboratory. They were given a cranial fenestration operation for an acute brainstem hemorrhage animal model and an enterstomy on the abdominal wall was made. Microcirculation microscopic blood vessel telecamera and microcirculation TV video system were used to continuously detect the congestion of the duodenal mucosal villi under brainstem hemorrhage conditions. Computer and photoshop software were used to continuously analyze the changes with time of the red color coordinate of the same villus on the same point. Result The red color (pallor) of duodenal mucosa reduced, along with minimal morphological changes of duodenal mucosa after brainstem hemorrhage. Conclusions Under the condition of this experiment, this surgical method can establish the experimental animal model to observe microcirculation of duodenal mucosal villi, and reduction of the red color coordinate with time verified the pallor of duodenal mucosa after brainstem hemorrhage in a rabbit model and reflected the sharp changes of mucosa's blood stream which is the most important preventive factor to stress ulcer under acute brainstem hemorrhage conditions. And these would become the pathophysiological basis of upper digestive tract ulcer.