Journal of Clinical Biochemistry and Nutrition
Online ISSN : 1880-5086
Print ISSN : 0912-0009
ISSN-L : 0912-0009
Original Articles
Inhibitory Effect of Lutein and Pycnogenol on Lipid Peroxidation in Porcine Retinal Homogenate
Takako Nakanishi-UedaMaki KamegawaSeiichiro IshigakiMasahiko TsukaharaSatoshi YanoKatsuya WadaHajime Yasuhara
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2006 Volume 38 Issue 3 Pages 204-210


This study aimed to investigate the antioxidant effect of lutein, human macula pigment, and pycnogenol, which contains several flavonoids, on lipid peroxidation induced in 10% porcine retinal homogenate by the addition of 1 mM Ferric chloride (FeCl3), 50 mM 2,2'-azobis (2-amidino-propane) dihydrochloride (AAPH), or 25 mM 2,2'-azobis (2,4-dimethyl-valeronitrile) (AMVN). Lipid hydroperoxide concentration was determined from the amount of thiobarbituric acid reactive substances (TBARS) in the sample following treatment. After 60 min of oxidation with FeCl3, AAPH and AMVN, the TBARS content in the retinal homogenates increased from 28.6 ± 1.6 to 85.4 ± 0.9, from 27.9 ± 1.2 to 57.2 ± 1.1, and from 26.0 ± 1.0 to 77.5 ± 2.0 nmol MDA/mg protein, respectively. Lutein did not show remarkable antioxidant activity in this experimental system. However, IC50 of pycnogenol for TBARS formation was decreased by combining 10 μM lutein in each initiator; from 12 to 5 μg/mL in FeCl3, from 2.8 to 0.5 μg/mL in AAPH, from 465 to 110 μg/mL in AMVN. These results suggested that a combination treatment of lutein and pycnogenol is more effective for inhibiting lipid peroxidation in porcine retinal homogenate. This synergy might be due to efficient functional antioxidants acting in both hydrophilic and lipophilic cellular environments.

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© 2006 by The Editorial Secretariat of JCBN
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