Role of Linoleic Acid Hydroperoxide Preformed by Cyclooxygenase-1 or -2 on the Regulation of Prostaglandin Formation from Arachidonic Acid by the Respective Enzyme

Abstract

Linoleic acid (LA) preincubated with cyclooxygenase (COX)-1 or -2 inhibited prostaglandin (PG) formation from arachidonic acid (AA) catalyzed by the respective enzyme, but LA without the preincubation did not. 13S-Hydroperoxy-9Z,11E-octadecadienoic acid (13-HPODE) a hydroperoxy adduct of LA inhibited PG formation catalyzed by COX-1 or -2. 13S-Hydroxy-9Z,11E-octadecadienoic acid had no effect on both COX-1 and -2 activities. These results suggest that 13-HPODE which is preformed from LA by COX reaction under normal physiological conditions can be a basal suppressor of PG formation from AA.