β-Elemene inhibits adipogenesis in 3T3-L1 cells by regulating AMPK pathway

Abstract

The prevalence of childhood obesity in global is quickly augmented, resulting into grievous public health problems and ‍influencing adolescent development. β-Elemene is a sesqui­terpene, and can extracted from traditional Chinese medicine-Curcuma longa L. β-Elemene has been discovered to display regulatory functions in multiple diseases, but it’s roles in obesity need further investigations. The purpose of this work is to investi­gate the regulatory impacts of β-elemene on obesity progression and associated pathways. In this study, it was revealed that the ‍heightened lipid accumulation in 3T3-L1 cells triggered by 3-‍isobutyl-1-methylxanthine + dexamethazone + insulin (MDI) can be restrained by β-elemene. Furthermore, β-‍elemene can modulate lipid metabolism in 3T3-L1 cells mediated by MDI. The glucose consumption was descended after insulin resistance treatment, but this impact was reversed after β-elemene treatment. At last, it was illustrated that the AMPK pathway was retarded after β-elemene induction, but this change was offset after β-elemene treatment. To sum up, our results manifested that β-elemene inhibited adipogenesis in 3T3-L1 cells, and evoked the AMPK pathway. This project may supply serviceable insights of β-elemene in the progression of obesity.