抄録
Effects in sedentary and exercised rats of allopurinol (a xanthine oxidase activity inhibitor) administration on lipid peroxidation and antioxidants were investigated by measurement of malondialdehyde (MDA), as well as α-tocopherol, β-tocopherol, γ-tocopherol, and total glutathione, concentration in plasma and liver, and comparison with those in untreated sedentary and exercised rats. Liver lipid peroxide concentration as measured by MDA in the exercised control group was significantly (p<0.05) increased by 104.2% compared with that of the sedentary control group. In the allopurinol-treated groups, the liver lipid peroxide concentration was 57.0% greater in the exercised group than in the sedentary group. Plasma lipid peroxide concentration in the exercised control and exercised allopurinol-treated groups increased over those of the respective sedentary groups. Plasma tocopherol (vitamin E) levels were greater in exercised groups than in corresponding sedentary groups. However, liver vitamin E levels of exercised control rats were significantly (p<0.05) decreased compared with those of sedentary control rats. Plasma total glutathione level in the exercised control group was decreased compared with that in the sedentary control group, and liver total glutathione level in the exercised control group was significantly (p<0.01) decreased compared with that in the sedentary control group. These results suggest that intensive exercise induced greater generation of oxygen radicals and enhanced lipid peroxidation in blood and liver. In addition, these results indicate that antioxidants as vitamin E located in cell membranes may be consumed by reactions that inhibit lipid peroxidation following exercise. This response to allopurinol treatment indicates that sources of oxygen radicals are not only the electron transport system in mitochondria but also the activation of xanthine oxidase during intensive exercise.
