Abstract
Effect of a water-soluble benzoquinone derivative, 4-[4-N-(5-ethyl-1-thia-3, 4-diazol-2-yl)sulfophenylamino]-5-methoxy-1, 2-benzoquinone, on the injury to cultured aortic endothelial cells induced by linoleic acid hydroperoxide was examined. The benzoquinone derivative markedly inhibited the release of lactate dehydrogenase(LDH) from the cells into the medium in a concentration-dependent manner. The inhibitory effect of the benzoquinone derivative tended to be higher than that of butylated hydroxytoluene. Upon incubation of the endothelial cells with the hydroperoxide, the lipid peroxide level in the cells was increased prior to the release of LDH from the cells, and this increase was significantly inhibited by the novel antioxidant.
