抄録
We studied the influence of semiconductor laser irradiation on the cultured human epidermal keratinocytes and measured cell viability and the concentration of interleukin-1α (IL-1α) in the medium of irradiated cell cultures. We found that the number of viable cells in the cultures was significantly increased by giving semiconductor laser irradiation for 15s (115.0±0.5%), 20s (112.0±1.0%) compared with the number in the nonirradiated cultures (p<0.01). The production of IL-1α by cultured cells was increased by the laser irradiation for 20s (35.8±5.5pg/ml) compared with the control (27.0±5.6pg/ml) after a 12-h incubation period (p<0.01). These results imply that low-power laser irradiation has significant biological effects on human keratinocytes.
