Journal of Clinical Biochemistry and Nutrition
Online ISSN : 1880-5086
Print ISSN : 0912-0009
ISSN-L : 0912-0009
Peroxisomal Proliferation in Rat Liver and Essential Fatty Acid Deficiency
Eric RABETAFIKAJean-Paul CARREAUClaude LE GOASCOGNE
著者情報
ジャーナル フリー

2002 年 31 巻 p. 27-36

詳細
抄録

The effects of essential fatty acid (EFA) deficiency were studied on male Wistar rats membrane lipid composition, enzyme activities and proliferation of liver peroxisome by feeding the animals, during 4 weeks, with fat free diets supplemented or not with a peroxisome proliferator, clofibrate (CLO).
In deficient (E) goup, peroxisomal membrane was characterized by higher level of total de novo synthesized unsaturated (n-9 and n-7 series) fatty acids and lower content of EFA (n-6 and n-3 series) and exhibited cholesterol (CHOL) to phospholipids (PLs) ratio diminution compared with that of control (C) group. Co-administration of CLO with the fat-free diet (EC group) enhanced the membrane uptake of n-9 fatty acids. Slight increase of membrane dihydroxyacetone-phosphate acyl-transferase (DHAP-AT) and decrease of palmitoyl-CoA synthetase activities were found in E group which featured normal-shaped peroxisomes.
In EC group, enzyme inductions of peroxisomal pamitoyl-CoA oxidase and palmitoyl-CoA synthetase were slightly higher and significantly lower respectively compared with those of C group treated with CLO (CC group) whereas activity of DHAP-AT was stable. Identical marked proliferation of peroxisomes were observed in both groups.
It is inferred that, despite the parallel alterations of membrane lipid composition and of enzyme activities of peroxisomes upon 4 weeks of EFA deficientdiet, the biogenesis and proliferation of the organelles were independently processed at a normal level. The possibility of homeostatic regulation and its physiological importance were discussed.

著者関連情報
© The Editorial Secretariat of JCBN
前の記事 次の記事
feedback
Top