1988 年 4 巻 1 号 p. 73-86
Nerve growth factors (NGF's) were purified from mouse submaxillary gland, guinea pig prostate gland, bovine seminal plasma, and venom of Naja naja atra, and their biochemical and immunological properties were compared. The NGF's from the four sources stimulated the maximal response of nerve fiber outgrowth from chick dorsal root ganglia at 10ng/ml. Two-site enzyme immunoassay (EIA) systems for these NGF's showed that mouse NGF, guinea pig NGF, and bovine NGF were similar in immunological properties and that the crossreactivity was less than 1% between snake NGF and mammalian NGF's. However, pretreatment with anti-snake NGF antibody inhibited the biological activity of mammalian NGF's even when their immunological activity was not completely inhibited. These results suggest that snake NGF and mammalian NGF's are similar in their biological activity but not in their immunological activity and that anti-snake NGF antibody recognizes the parts of mammalian NGF's essential for the biological activity.
