What Do Thiobarbituric Acid and Hemoglobin-Methylene Blue Tests Evaluate in the Endogenous Lipid Peroxidation of Rat Liver?

抄録

To detect the endogenous lipid peroxides in rat liver, the thiobarbituric acid (TBA) and hemoglobin-methylene blue (HMB) tests were used. The endogenous lipid peroxidation in rat liver was induced by peroxidative drugs, and then these tests were carried out. When CCl₄, paraquat or phenobarbital was intraperitoneally injected, the HMB values were significantly increased only at the early period after the doses (1h), whereas two peaks of TBA value were observed at 1h and around 24h after the doses. When peroxidation was induced by thiopental or alcohol, the TBA value also gave two peaks. It was suggested that the agreement between detections by both HMB and TBA (the early peak) indicated the peroxide formation, and that the independent increase in TBA value (second peak) the accumulation of aldehydes in the liver. The life span of HMB-reactive substances was short in vivo compared with that of the TBA-reactive substances. It is considered that the TBA test was useful to detect the endogenous lipid peroxidation under the conditions that TBA test could detect aldehydes originating from lipid peroxidation because lipid peroxides were unstable in vivo.