1989 年 7 巻 2 号 p. 81-89
A relatively simple method for purification of human platelet-derived growth factor (PDGF) was developed. PDGF was purified from clinically outdated, platelet-rich plasma by means of freezing and thawing extraction and successive chromatography on CM-Sephadex, Sephacryl S-200, and Phenyl Sepharose. Further purification of the PDGF obtained showed two silver-stained bands following polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate (SDS-PAGE). Amino acid sequence analysis of these two components separated by SDS-PAGE demonstrated that the sequences coincided with those of PDGF A and B chains previously reported and predicted from the nucleotide sequences of the cloned cDNA's of PDGF A and B chain genes. The purified PDGF preparation stimulated a nanogram level of thymidine incorporation into DNA of previously quiescent Balb/c 3T3 cells.
