Quantitative Proteomic Analysis System: i-OPAL

抄録

Recently, significant improvements have been shown in proteomics, an analytical technology recognized as a method to discover biomarkers. Several methods for proteome differential display have been developed to discover biomarkers, and these are based on labeling methods such as ICATÔ reagent. These labeling methods are not easily able to detect biomarkers in complex biological samples, and they have several issues such as the limitation of the modification site and the production of artificial errors. We therefore developed a computational quantitative proteomic analysis system i-OPAL (internal standard guided Optimal Profile ALignment) based on LC-MS measurements for biomarker discovery. This system can provide extensive quantitative analysis because it does not involve any artificial labels that might cause sampling bias and limitation of quantifiable proteins.