2021 年 54 巻 7 号 p. 351-357
Industrial/commercial-scale biological applications including cell therapy, drug testing and regenerative medicine, require the production of a large number of cells. A suspension culture method has been extensively used to generate sufficiently large quantities of high-quality cells in a defined form. In this study, we carried out the present numerical simulation to first characterize the performance of two different shaking methodologies in an orbitally shaken bioreactor, namely, one direction rotation (ODR) and periodic alternate rotation (PAR). Cell distribution, suspension level and shear stress at varying shaking conditions are obtained, indicating that the PAR method is superior to another one, as it could generate upward fluid motion to suspend adequate cells. Additionally, the parametric study of the PAR method is further explored to capture an optimum culture condition concerning cells quality and energy consumption. It is concluded that our simulation results give a new insight for bioreactor design and would be beneficial to the shaking techniques for cell cultivation in suspension culture.
