抄録
Bone marrow-derived mesenchymal stromal cells (BMSCs) are pluripotent progenitor cells that can regenerate different skeletal tissues in response to environmental signals. Scaffolds play a critical role in tissue engineering, and their microstructure is essential for inductive bone formation. In the present study, we have used highly porous, structurally stable three-dimensional apatite-fiber scaffolds (AFSs) and investigated their ability to support cell proliferation and differentiation. BMSCs in/on AFSs were proliferated in a three-dimensional manner when both micro- and macro-pores were present. The characteristic structure of the AFS enhanced calcification, production of extracellular matrix (ECM), and osteogenic differentiation, especially endochondral ossification. Furthermore, BMSCs cultured in/on AFSs did not undergo adipogenesis and selectively underwent osteogenic differentiation. These results suggest that AFSs provide a suitable environment for bone regeneration and have potential applications for tissue engineering.
