Development of a Calcium Imaging System for Mouse-derived Myoblast Cell Line C2C12 under Ultrasound Stimulation

Abstract

To optimize the irradiation conditions of ultrasound for medical applications, a system that enables real-time observation of cellular responses to ultrasound is required. This study hypothesizes that cells exhibit frequency-dependent responses to ultrasound and aims to verify this. We propose a system with a cylindrical structure for real-time observation and an amplitude-enhancing mechanism that accommodates multiple driving frequencies. Using the fabricated system, we applied ultrasound at two different frequencies to cells and observed calcium influx. The results suggest that the vibration velocity at which calcium influx occurs may depend on the ultrasound frequency.