The gold clusters, less than 50 A in diameter, became structurally unstable under the electron beam irradiation (105 electrons/A2. sec) and continually changed their shapes; single, twinned and multiply-twinned crystals. Two possibilities for causing the instability can be thought; specimen temperature rise or surface charging due to secondary electron emission. Electron microscope observation utilizing a fine-focused electron beam favors to the surface charging effect. Reorganization of atoms in the clusters, often undergoing in the twin deformation, was caused by a shear stress due to a coulomb force arising from the positive surface charges.