抄録
In the course of the writer's experiments with self-incompatible lines of Petunia violacea, it was noticed that the germination of pollen-grains and the growth of pollen-tubes were inhibited by the stigma-secretion, or the tissue-juice of styles, or both. Although it is very interesting to know the exact time when these substances are produced, yet it can not be definitely stated as yet. So far as the writer's experiments go, it may be conjectured that they may be produced shortly before or after the opening of flowers. So, the pollinations between the pistils of the flower-buds of self-incompatible individuals of Petunia violacea and the pollens of open flowers of the same plant was made as well as between the pistils and pollens of open flowers of the same individual. The result of such experiments were that pistils of the flower-buds were easily fertilized, but those of open flowers were utter failure in their fertilization. The seeds thus ontained germinated fairly well. The experiments and their results above noticed show the posibility of obtaining pure-line seeds of self-incompatible plants, at least in petunia violacea, by means of the pollination method above stated, i. e., by carrying on the pollination between the pistils of flower-buds and the pollens of open flowers.
