日本作物学会紀事
Online ISSN : 1349-0990
Print ISSN : 0011-1848
ISSN-L : 0011-1848
イネの培養遊離細胞における脂質体について
前田 英三
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ジャーナル フリー

1968 年 37 巻 4 号 p. 551-556

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A number of single cells and cell aggregates were produced from rice callus tissues in liquid medium at 25-30°C. The freely suspended living cells and aggregated cells were containing one or more of spherical granules, 2.8μ of average diameter. The granules disappeared with acetone, ethanol, ethylene glycol, formalin, isopropanol, pyridine, triethyl phosphate and chloroform methanol mixture. They were also stained blue by 1% or 0.02% Nile blue. But the colour was not removed by 5% acetic acid or 0.5% hydrochloric acid. From these results, it is revealed that the granules are composed of liquid, acidic lipids.
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