Streptococcus sobrinus SL 1株のgtf遺伝子のクローニング
1993 年 43 巻 1 号 p. 104-112
詳細
1993 年 43 巻 1 号 p. 104-112
The gtf genes coding for glucosyltransferase (GTF), which produces water-soluble glucan, were cloned from Streptococcus sobrinus SL1 (ATCC 33478).
Chromosomal DNA of S. sobrinus SL1 was digested with Pst I and ligated into Pst I-cleaved plasmid vector pUC19. After transformation, a clone library was generated.
Through the screening of the clone library, two sucrase-positive transformed cells were selected from 213 transformants. One sucrase-positive clone carried plasmid pYTK 100, consisting of an insert of a 6.1kb Pst I-Pst I fragment containing the gene which expressed a glucosyltransferase synthesizing a water-soluble glucan. The gene inserted in pYTK 100 was very similar to gtfT gene of S. sobrinus OMZ176 previously isolated. Another sucrase-positive clone carried plasmid pYTK 104, consisting of an insert of a 4.5kb Pst I-Pst I fragment, which contained a part of a fourth gtf gene (gtfU) of S. sobrinus.
