原著
Enterococcus faecalisが形成するバイオフィルムに対する中性電解機能水パーフェクトペリオ®の抗菌効果に関する基礎的研究
2010 年 31 巻 1 号 p. 29-35
詳細
2010 年 31 巻 1 号 p. 29-35
Abstract : The aim of this study was to investigate the antimicrobial effects of electrolyzed neutral water (Perfect Perio®) (PPW) on Enterococcus faecalis formed biofilm. This PPW contained hypochlorous acid concentrations at 600-700 ppm in pH 7.5. The effects of PPW were compared with those of NaClO and sterilized water (DW). Biofilms of E. faecalis were induced on tissue-culture plates. An overnight culture of E. faecalis grown in brain-heart-infusion broth was seeded (initial concentration of 107-8 cells/mL) with trypticase soy broth (with 0.25% glucose), which was incubated under aerobic conditions for 48h to allow biofilm formation. After incubation, the biofilms were irrigated with PBS and treated as irrigants. The remaining biofilms were stained with crystal violet to gratify the amounts of biofilm, which were determined using a microplate reader. Morphological changes of E. faecalis biofilm by NaClO, PPW or DW were investigated by SEM.
NaClO can disaggregate and remove biofilm at all times, and treatment with PPW can cause a high degree of biofilm disaggregation. SEM analysis showed that 5% NaClO eliminated the bacteria completely, PPW was capable of disrupting and removing the biofilm, but not eliminating the bacteria.
According to the results, PPW showed the highest eliminatory effect on the E. faecalis-derived biofilm.
