抄録
We have developed a method for the determination of chlorobenzenes in blood by head space SPME-GC/MS. Five ml of blood sample is put into a head space bottle, to which an enzyme is added for proteolysis. The head space of the bottle is sampled by a solid phase micro extraction (SPME) . The SPME fiber is analyzed for chlorobenzenes by GC/MS. This method is excellent in sensitivity and selectivity, easy to operate, and is applicable to actual samples. This is also an environmentally friendly method, for it requires only small amounts of reagents and solvents.
Blood samples were collected from 60 subjects, all of whom are members of Nagano Research Institute for Health and Pollution and their families, and analyzed. In all the blood samples, p-dichlorobenzene (0.4-210 ppb; 14.9 ppb on the average) and HCB (0.07-0. 40 ppb; 0.17 ppb on the average) were detected. p-Dichlorobenzene is considered to have been derived from moth-proofing agents for clothes and odor-removing agents for toilets, whereas HCB is considered to have been derived from food.
