New Method of Specimen Preparation as Cell Suspension for a Micronucleus Test

Abstract

A new specimen preparation method named “cell suspension method” for in vivo micronucleus test is proposed. Generally, a specimen of the micronucleus test is prepared by smearing the sample cells on a slide glass before staining with Giemsa or acridine orange (A.O.). In this new method, the sample cells are suspended with formalin, and stained with A.O., then put on a slide glass and examined under a microscope without smearing. This method is more convenient than the conventional method in consideration of preparation and observation of specimens. Furthermore, preparation is repeatable after the observation in this method, the specimen is suitable for a long storage, and this method is applicable to specimen preparation using peripheral blood.