1972 年 1972 巻 9 号 p. 29-34
An acute “coughing disease” broke out among racehorses and riding ones in Japan in December, 1971. In this outbreak, affected horses showed the same symptoms, including fever at 39-41°C, dry cough characterized by stregth, and the secretion of nasal mucus . The disease was suspected to be equine influenza from clinical symptoms. The direct fluorescent antibody technique was applied to some horses involved. The results obtained are summarized as follows. 1.The nasal secretion collected from affected horses was positive for direct fluorescent antibody. It was found that this epizootic had been caused by equine influenza which had never occurred in Japan. 2.Of 29 samples collected from 5 epizootic regions, 18 samples (62.1 %) were proved to be positive. 3.The direct fluorescent antibody technique was considered to be extremely effective for the early diagnosis of equine influenza and for the prevention of the disease. 4.The positive cells were composed mainly of round epithelial cells containing fluorescenn in the cytoplasm . There were 4-8 positive cells perten thousand normal epithelial cells in the direct nasal smear.