馬鼻肺炎生ウイルスワクチンの研究
1. ウシ腎細胞継代弱毒株の馬に対する病原性
1978 年 1978 巻 15 号 p. 31-36
詳細
1978 年 1978 巻 15 号 p. 31-36
To develop a live equine rhinopneumonitis virus (ERV) vaccine for the prevention of upper respiratory infection in colts, an attempt was made to attenuate a strain of ERV HH1 by making 320 passages in BK cell cultures. In growth experiments, the attenuated strain, HH1-BK-320, showed a very high titer when incubated at 30°C, but hardly multiplied a 40°C. CPE was observed at 48 hours of incubation, when the infective titer of the virus against BK cell culture ranged from 106.5 to 107 TCID50. A colt was inoculated intranasally with the attenuated virus. No characteristic respiratory signs were observed, but a small amount of virus was detected in nasal washings a short time after inoculation. After that, the colt showed a significant rise in titer of neutralizing antibody against the original HH1 strain. From these results, it was presumed that the HH1-BK-320 strain might be safe enough for use as live virus vaccine for colts.
