2013 Volume 59 Issue 2 Pages 135-140
A novel strain of Enterobacter, C2361T, a Gram-negative, non-spore-forming, rod-shaped and facultative anaerobic bacterium with the capability to produce transglutaminase, was isolated from seafood processing wastewater collected from a treatment pond of a seafood factory in Songkhla Province, Thailand. Phylogenetic analyses and phenotypic characteristics, including chemotaxonomic characteristics, showed that the strain was a member of the genus Enterobacter. The 16S rRNA gene sequence similarities between strain C2361T and Enterobacter cloacae subsp. cloacae ATCC 13047T and Enterobacter cloacae subsp. dissolvens LMG 2683T were 97.5 and 97.5%, respectively. Strain C2361T showed a low DNA-DNA relatedness with the above-mentioned species. The major fatty acids were C16:0, C17:0cyclo and C14:0. The DNA G+C content was 53.0 mol%. On the basis of the polyphasic evidence gathered in this study, it should be classified as a novel species of the genus Enterobacter for which the name Enterobacter siamensis sp. nov. is proposed. The type strain is C2361T (= KCTC 23282T = NBRC 107138T).