The Journal of General and Applied Microbiology
Online ISSN : 1349-8037
Print ISSN : 0022-1260
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A novel small regulatory RNA enhances cell motility in enterohemorrhagic Escherichia coli
Naoki SudoAkiko SomaAkira MutoSunao IyodaMayumi SuhNanako KuriharaHiroyuki AbeToru TobeYoshitoshi OguraTetsuya HayashiKen KurokawaMakoto OhnishiYasuhiko Sekine
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2014 Volume 60 Issue 1 Pages 44-50

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Abstract

Small regulatory RNAs (sRNAs) are conserved among a wide range of bacteria. They modulate the translational efficiency of target mRNAs through base-pairing with the help of RNA chaperone Hfq. The present study identified a novel sRNA, Esr41 (enterohemorrhagic Escherichia coli O157 small RNA #41), from an intergenic region of an enterohemorrhagic E. coli (EHEC) O157:H7 Sakai-specific sequence that is not present in the nonpathogenic E. coli K-12. Esr41 was detected as an RNA molecule approximately 70 nucleotides long with a 3′ GC-rich palindrome sequence followed by a long poly(U), which is a characteristic of rho-independent terminators and is also a structural feature required for the action of Hfq. EHEC O157 harboring a multicopy plasmid carrying the esr41 gene increased cell motility and the expression of fliC, a gene encoding a major flagellar component. These results indicate that Esr41 stimulates fliC expression in EHEC O157. Furthermore, the increase in cell motility induced by Esr41 was also observed in the E. coli K-12, suggesting that target genes controlled by Esr41 are present in both EHEC O157 and K-12.

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© 2014, Applied Microbiology, Molecular and Cellular Biosciences Research Foundation
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