The Journal of General and Applied Microbiology
Online ISSN : 1349-8037
Print ISSN : 0022-1260
Full Papers
A novel small regulatory RNA enhances cell motility in enterohemorrhagic Escherichia coli
Naoki SudoAkiko SomaAkira MutoSunao IyodaMayumi SuhNanako KuriharaHiroyuki AbeToru TobeYoshitoshi OguraTetsuya HayashiKen KurokawaMakoto OhnishiYasuhiko Sekine
Author information

2014 Volume 60 Issue 1 Pages 44-50


Small regulatory RNAs (sRNAs) are conserved among a wide range of bacteria. They modulate the translational efficiency of target mRNAs through base-pairing with the help of RNA chaperone Hfq. The present study identified a novel sRNA, Esr41 (enterohemorrhagic Escherichia coli O157 small RNA #41), from an intergenic region of an enterohemorrhagic E. coli (EHEC) O157:H7 Sakai-specific sequence that is not present in the nonpathogenic E. coli K-12. Esr41 was detected as an RNA molecule approximately 70 nucleotides long with a 3′ GC-rich palindrome sequence followed by a long poly(U), which is a characteristic of rho-independent terminators and is also a structural feature required for the action of Hfq. EHEC O157 harboring a multicopy plasmid carrying the esr41 gene increased cell motility and the expression of fliC, a gene encoding a major flagellar component. These results indicate that Esr41 stimulates fliC expression in EHEC O157. Furthermore, the increase in cell motility induced by Esr41 was also observed in the E. coli K-12, suggesting that target genes controlled by Esr41 are present in both EHEC O157 and K-12.

Information related to the author
© 2014, Applied Microbiology, Molecular and Cellular Biosciences Research Foundation
Previous article