The Journal of General and Applied Microbiology
Online ISSN : 1349-8037
Print ISSN : 0022-1260
ISSN-L : 0022-1260
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An efficient shortened genetic transformation strategy for filamentous fungus Trichoderma reesei
Chuan WuYumeng ChenXiaoxue HuangShishuai SunJinnan LuoZhiwen LuWei WangYushu Ma
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2019 Volume 65 Issue 6 Pages 301-307


The filamentous fungus Trichoderma reesei is one of the most important fungi for the production of cellulases and xylanases, which can be used for biofuel production from lignocellulose. We aimed to develop an effective selection marker system for more extensive functional genomic studies in the fungus T. reesei, and to construct better industrial transformants for producing cellulases. Here, we present a novel effective G418 selection marker to use a codon-optimized neomycin phosphotransferase II gene nptII to transform T. reesei. We developed an effective and erasable selection marker, lcNG, and a combined genetic transformation system for gene manipulation in T. reesei using a two-Agrobacterium-mediated transformation method. This transformation strategy combines two steps in the transformation protocol, which saves 15–30-day's time. The system could be a useful tool for the genetic engineering of T. reesei.

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© 2019, Applied Microbiology, Molecular and Cellular Biosciences Research Foundation
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