QUANTITATIVE STUDIES OF ENZYME SYSTEM OF HETEROLACTIC FERMENTATION

I. SOME ASPECTS OF CYTOPLASM OF LEUCONOSTOC MESENTEROIDES AS THE SITE OF HETEROLACTIC FERMENTATION

Abstract

As a preliminary step of quantitative studies of the heterolactic fermentation system, standard conditions for steady rate fermentation were set up. Some basic aspects of the cytoplasm of Leuconostoc mesenteroides were studied under the defined conditions. These included dimension of the cytoplasm, internal pH, and concentration of several inorganic ions. Distribution and levels in the cells of NAD, NADP, thiamine pyrophosphate and CoA were also studied. Cytoplasmic levels of ATP and ADP were followed and found to be constant during fermentation.