MICROBIAL PRODUCTION OF AMINO ACIDS FROM HYDROCARBONS

II. ISOLATION OF GOOD HYDROCARBON UTILIZERS AND AMINO ACID PRODUCTION BY THEIR AUXOTROPHS

Abstract

Nine good hydrocarbon utilizers including Corynebacterium hydrocarboclastus, Alcaligenes marshallii and yeasts were isolated. They showed abundant growth on a simple synthetic hydrocarbon medium after 24-hr incubation.
Various auxotrophic mutants derived from them produced the following amino acids: Ornithine, valine, glutamic acid, leucine, tyrosine, alanine, proline, aspartic acid, and lysine.
The anticipation of this method for fermentative production of amino acids from hydrocarbons was also discussed.