The Journal of General and Applied Microbiology
Online ISSN : 1349-8037
Print ISSN : 0022-1260
ISSN-L : 0022-1260
TRANSFORMATION IN BACILLUS SUBTILIS
II. INFLUENCE OF A PROTEIN FRACTION BOUND TO DNA ON THE TRANSFORMING ACTIVITY
ONOFRIO LOSTIAANGELO CARERERUBENS LOPEZ GARCIACESARE ROSSI
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1972 Volume 18 Issue 4 Pages 263-269

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Abstract

DNA of B. subtilis, extracted according to Marmur but deproteinized in the apparatus described in the preceding paper of this series, has a higher transforming efficiency and a higher percentage protein content than DNA obtained exclusively by the Marmur method. Experiments were carried out in order to establish whether the higher transforming ability was due to the higher residual content of proteins. Results obtained by the action of Pronase on both samples of DNA support this hypothesis.

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