MUTABILITY OF THE PHLEOMYCIN-RESISTANT MUTANTS OF BACILLUS SUBTILIS

II. DNA SYNTHESIS AND PHAGE GROWTH IN A MUTABLE STRAIN

Abstract

DNA-synthesizing activity of a mutable strain, MU-10, selected from the phleomycin-resistant mutants was investigated in comparison with that of parental strain, 160 trp. The DNA-synthesizing machinery in MU-10 was more sensitive to high temperature and more resistant to phleomycin than that in 60 trp and, moreover, the mutant did not allow the full synthesis of phage DNA although the cell took up the phage genome. The mutator gene, mut, was unseparable by genetic means from the phleomycin-resistant gene (phl).