Abstract
We report here the action of the β-glucosidase, nitrite hydratase and amidase of Brevibacterium sp. R 312 during the in vitro degradation of some cyanoglucosides. The degradation pathway found for prunassin begins with the action of the β-glucosidase followed by the action of the nitrite hydratase and of the amidase, but linamarin can also be attacked first by the nitrite hydratase. The Vm and Km of the nitrite hydratase for the main nitrite compounds and of the β-glucosidase for cyanoglucosides were determined with partially purified extracts.
