INDUCTION OF NUTRITIONAL MUTANTS OF GLUTAMIC ACID BACTERIA AND THEIR AMINO ACID ACCUMULATION

Abstract

Many auxotrophic mutants were obtained from glutamic acid bacteria by UV, Co⁶⁰ irradiation applying penicillin selection method. Mutants which accumulate ornithine, lysine, valine, homoserine, phenylalanine, diaminopimelic acid, α-aminobutyrate, tyrosine were found. Close relationships between accumulated amino acids and requiring substances were suggested.
Lysine accumulating strains obtained in the present study are now being used in commercial production of lysine. The possibility of the application of accumulation of other metabolite by auxotrophs to industrial production was also discussed.