Simple Preparation and Decoloration Ability of Immobilized Soybean Peroxidase PET Fabric
2020 Volume 71 Issue 12 Pages 766-774
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2020 Volume 71 Issue 12 Pages 766-774
Peroxidase in soybean root extract (SPO) was immobilized on a simple amine-treated PET fabric and used to decolorize dye in wastewater following fabric dying.
Treating PET fiber with 50 wt％ ethylenediamine resulted in a small weight loss of the fabric and introduced amino groups.
We investigated SPO immobilization conditions on the EDA-treated PET (EDA-PET) fabric and found that the optimum pH for SPO immobilization was pH 4, and the optimum buffer was Carmody buffer using an immobilization temperature of 20℃.
Examination of the conditions for the decoloration of Orange I with SPO immobilized PET fabric (SPO-EDA-PET) indicated that the optimum concentration of p-coumaric acid was 5.0×10-4 M, the optimum concentration of hydrogen peroxide was 8.3×10-4 M, and the optimum reaction temperature was 20℃.
Repeating the decoloration reaction of Orange I with SPO-EDA-PET four times provided a reaction rate constant of 1.12×10-3 min-1, which is adequate for practical wastewater treatment.
