Abstract
The chemical structure of glycopeptide of mucilage in Hibiscus esculentus (Okura) was identified by chromatographies (thin layer, ion exchange resin and paper) -, dinitrophenylation-, enzymatic hydrolysis- and Edman degradation-techniques.
Peptide containing carbohydrate had glycine and serine as the N-and C-terminal amino acid residues, respectively.
The composition of the peptide was glycine, aspartic acid (or asparagine), threonine, serine and glutamic acid (or glutamine) as the amino acids, and glucose and fructose as the carbohydrates. The fact that threonine is located next to aspartic acid or asparagine in the peptide chain was discussed comparing with certain other glycopeptides. The identified amino acid sequence of peptide moiety of this glycopeptide in Okura was ; Gly-Asx-Thr-Ser-Glx-Ser.
